论文题目:A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis
论文作者：Yudi Wang#, Yongdong Li#, Zonglan Yu, Wenjing Zhang, Zhaorui Ren, Hongyun Zhang, Lu Xiao, Shengzhi Guo, Jinfang Zhou, Lingfang Zhu, Changfu Li, Lei Xu, Yao Wang, Yi Chen, Xihui Shen*, Yantao Yang*
论文摘要：
The bacterial type VI secretion system (T6SS) mediates interactions with neighboring cells or the environment by secreting various effectors, yet T6SS RNase effectors have barely been studied. Here, we report a multifunctional trans-kingdom T6SS RNase effector, TseR, from Yersinia pseudotuberculosis (Yptb). TseR, an Ntox44 domain-containing effector secreted by T6SS-3, is a divalent cation-dependent RNase that preferentially cleaves single-stranded RNA, which can be inhibited by the cognate immunity protein TsiR. TseR mediated both contact-dependent and contact-independent T6SS killing of bacteria, with OmpC facilitating its entry into target cells during contact-independent killing. The global cleavage targets of TseR in Escherichia coli were identified via RNA-seq analysis. During infections, TseR facilitated pathogenicity by altering the gut microbiome and directly targeting eukaryotic host cells. This study provides valuable insights into the roles of the T6SS RNase effector and the Ntox44 family protein in bacterial competition and bacteria-host interactions.
细菌VI型分泌系统（T6SS）通过分泌各种效应蛋白介导与周围细胞或环境的相互作用，然而T6SS RNase效应蛋白鲜有研究。在这里，我们报告了一种来自假结核耶尔森氏菌（Yersinia pseudotuberculosis, Yptb）的多功能跨界型T6SS RNase效应蛋白TseR。TseR是一种由T6SS-3分泌的含有Ntox44结构域的二价阳离子依赖型RNase效应蛋白，它优先切割单链RNA，可以被同源免疫蛋白TsiR抑制。TseR介导了接触依赖型和接触非依赖型两种T6SS杀菌作用，在接触非依赖杀菌过程中OmpC辅助TseR进入靶标细胞。我们还通过RNA-seq分析确定了TseR在大肠杆菌中的全局切割靶标。在感染过程中，TseR通过改变肠道微生物组并直接靶向真核宿主细胞来促进该菌的致病性。这项研究为T6SS RNase效应蛋白和Ntox44家族蛋白在细菌竞争和细菌-宿主相互作用中的功能提供了宝贵的见解。
原文链接：https://www.cell.com/cell-reports/fulltext/S2211-1247(25)00845-9?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2211124725008459%3Fshowall%3Dtrue